The vitamin K cycle
نویسندگان
چکیده
منابع مشابه
Functional study of the vitamin K cycle in mammalian cells.
We describe a cell-based assay for studying vitamin K-cycle enzymes. A reporter protein consisting of the gla domain of factor IX (amino acids 1-46) and residues 47-420 of protein C was stably expressed in HEK293 and AV12 cells. Both cell lines secrete carboxylated reporter when fed vitamin K or vitamin K epoxide (KO). However, neither cell line carboxylated the reporter when fed KO in the pres...
متن کاملA cellular system for quantitation of vitamin K cycle activity: structure-activity effects on vitamin K antagonism by warfarin metabolites.
Warfarin and other 4-hydroxycoumarins inhibit vitamin K epoxide reductase (VKOR) by depleting reduced vitamin K that is required for posttranslational modification of vitamin K-dependent clotting factors. In vitro prediction of the in vivo potency of vitamin K antagonists is complicated by the complex multicomponent nature of the vitamin K cycle. Here we describe a sensitive assay that enables ...
متن کامل[Vitamin K].
In this paper, highly sensitive methods for measuring of vitamin K in serum, feces and milk are described. Vitamin K1 and the menaquinone family from the serum, feces and milk were separated by high performance liquid chromatography. The separated K1 and menaquinone families were detected by a fluorescence spectrophotometer after their reduction with sodium borohydride or platinum catalyst colu...
متن کاملVitamin K
Vitamin K ................................................................................................................................... 1 1 Dietary sources and intake ..................................................................................................... 1 2 Physiology and metabolism ...............................................................................................
متن کاملVitamin K Metabolism and Vitamin K-Dependent Proteins
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ژورنال
عنوان ژورنال: Journal of Thrombosis and Haemostasis
سال: 2005
ISSN: 1538-7933,1538-7836
DOI: 10.1111/j.1538-7836.2005.01419.x